Journey times vary depending on traffic and road conditions, approximate travel times are:

 

From London (1-1.5 hours)
M40 Junction 7 towards Thame, via Long Crendon and Upper Winchendon

 

From Birmingham (1-1.5 hours)
M40 Junction 9 and take the A34 via Bicester, then follow A41 towards Aylesbury

 

From Oxford (30 mins)
A34 towards Bicester, then follow A41 towards Aylesbury

 

From Milton Keynes (45 mins)
A5 towards Dunstable, then A4146 via Leighton Buzzard to Aylesbury. Finally A41 towards Bicester